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Biology 200 Multiple Questions for Grade 12 Students

Prepared by: Yeshaneh Adimasu (Lecturer at Adama Science and Technology Uuniversity) 

                                              "Together We Can" 

There are #200 multiple questions which is very helpful for all grades, especially for Grade 12 students around every corner of Ethiopia. Th questions are prepared based on the New curriculum from Grade 11 (unit 1-3) and 12 (unit 1&2) and  old curriculum from Grade 9 (Unit 1-3) and 10 (Unit 1) 

 Those who have interest to access the video lectures from Grade 9, 10, 11, and 12, please visit my YouTube channel "Yeshaneh Tube" on the link: https://www.youtube.com/@yeshanehtube 

Further more; If you have any comment and suggestion please write on the comment section 

@yeshanehtube 

DO carefully !!

Good luck 

Instruction: Choose the correct answer from the given alternatives

1. A device that is used to separate solids from liquids where simple filtration is not adequate for the task is

A/ Centrifuge              B/ Petri dish                 C/ Theodolite                 D/ Autoclave

2. Which one of the following scholars support the idea of spontaneous generation?

A/ Joseph Lister                  B/ Needham         C/ Robert Kochi          D/ Spallanzani

3. Biological research is highly relevant to us all because

A/ Producing new crops that will help to feed an increasing pest

B/ Producing new antibiotics against AIDS

C/ Producing new vaccine that are more effective against viral diseases

D/ Help to maintain, expand and prevent exotic plant species 

4. Which one of the following is inorganic molecule?

A/ Water, calcium carbonate, carbon dioxide        B/ Water, sugar, calcium carbonate

C/ Sugar, fatty acid, amino acid                             D/ Carbon dioxide, amino acid, fatty acid

5. The carbon, oxygen, and hydrogen composition in the carbohydrates respectively are---

A/ 1:2:1 ratio             B/ 2:1:1 ratio              C 1:1:2 ratio            D/ Equal ratio

6. The hydrogen and oxygen atom in the water molecule are joined by

A/ Ether bond                B/ Covalent bond     C/ Ionic bond             D/ Hydrogen bond

7. One of the most important things in biotechnology is producing potential plant and animal products. This can be achieved by inserting new genetic information into existing cells in order to modify a specific organism for the purpose of changing its characteristics. This method is

A/ Transgenic organism        B/ Cloning method       C/ rDNA technology           D/ GMO

8. Which one is the best definition of modern biotechnology?

A/ Biotechnology is the use of microbes to change crops and animals and producing new products

B/ Biotechnology is the use of microorganisms to make fermented food product

C/ Biotechnology is a way of making and preserving foods and making alcoholic drinks

D/ Biotechnology is the use of microbes to produced making cheese and yoghurt 

9. Which one of the following statements is true?

A/ Aerobic respiration provides more energy than anaerobic respiration, allowing yeast cells to grow and reproduce

B/ Anaerobic respiration provides less energy than aerobic respiration, allowing yeast cells to grow and reproduce more quickly

C/ Yeast cells can’t survive for a long time in low oxygen conditions, and will break down all the available sugar to produce ethanol

D/ Yeast cells can survive for a long time in low oxygen conditions, but don not break down all the available sugar to produce ethanol

10. In the fermentation application, which one is true about yeast?

A/ Yeast can respire anaerobically in bread making, but respire aerobically to make alcoholic drink

B/ When the alcohol content occurs in large amounts it will stop fermentation but do not kill yeast

C/ When the alcohol content occurs in large amounts it will kill yeast but don’t stop fermentation D/ Yeast can respire aerobically in bread making, but respire anaerobically to make alcoholic drink

11. Which one of the following is true about making yoghurt?

A/ As the yeast break down the lactose in the milk, they produce lactic acid, which gives the yoghurt its sharp, tangy taste.

B/ The microbes that used in yoghurt processing help to prevent the growth of other bacteria that normally lead the milk bad.

C/ Yoghurt is formed by the action of fungi on the lactose in the milk.

D/ The lactic acid produced by the fungi causes the milk to clot and solidify into yoghurt

12. The yeast cell is reproduced by asexual budding which leads to the splitting of parent cell into two to form new yeast cells. How many cells does one gram of yeast contain?

A/ About 10 million         B/ About 25 million         C/ About 40 billion      D/ About 25 billion

13.  Which one of the following is true about the feature of industrial fermenter or vessel?

A/ Usually has an oxygen supply to remove the excess heat produced by microorganisms

B/ Has a stirrer for continuous monitoring of factors such as pH and oxygen level

C/ Has a water-cooled jacket to remove the excess heat produced by the respiring microorganisms

D/ Has a water-cooled jacket for maintaining temperature and food are distributed evenly through the culture

14. Biogas is a flammable mixture of gases, formed when microorganism break down plant material, or the waste products of animals, in anaerobic conditions. So, which one gas is the major component of biogas based on percentage next to methane?

A/ Hydrogen sulphide               B/ Hydrogen           C/ Water            D/ Carbon dioxide          

15. Based on the following stages of making and using biogas, which one is the correct order:

I. Bacteria break down the plant parts and sugars, producing methane and other gases

II. Dung or plant material is collected and put into a biogas digester

III. The biogas produced is piped into homes and burned to produce light or heat

IV. A mixed population of different types of bacteria is added

A/ II, IV, I, and III            B/ II, I, IV, and III          C/ IV, I, III, and II        D/ IV, III, I, and II

16. Which one of the following statements is correct?

A/ The cells of early animal embryos are specialized

B/ In many animals, the cells become unspecialized at very early in life

C/ When baby is born, most of the cells have become unspecialized for a particular job

D/ When a muscle cell divides by mitosis, it can only form more muscle cells

17. Which one of the following is the correct sequence of scientific methods? 

A/ Title -------Hypothesis------Background ------Experiment------ Results-----Conclusion

B/ Title------Background-------Hypothesis------Experiment------ Results-----Conclusion

C/ Background------- Title------Hypothesis------ Results-----Experiment------ Conclusion

D/ Background------- Title------Hypothesis------Experiment------ Results-----Conclusion

18. Who was the first scholar conducting experiment on fly to disprove abiogenesis theory?

A/ Fransisco Redi                      B/ Needham           C/ Spallanzani      D/ Johan Tyndall

19. The scientific method is more reliable than opinion based on personal observation because:

A/ Scientists are more reliable than other people

B/ Observation is not a valid scientific technique

C/ Scientific method always gives the correct answer

D/ Scientific method involves gathering information from controlled experiments to prove or disprove a hypothesis

20. Which one of the following statements is odd about the properties of microscope?

A/ Resolution is the ability to distinguish between two points that are close together

B/ If resolution is poor, they will merge into one point and the detail of the image will be limited

C/ TEM and SEM have higher resolution than optical microscopes

D/ TEM and SEM have higher resolution and low magnification optical microscopes

21. Which one of the following properties of water is important for the uptake of water molecule from the ground to other part of the plant?

A/ Universal solvent                                 B/ High heat of vaporization

C/ Cohesion and adhesion                        D/ High specific heat capacity  

22. The high surface tension of water is due to:

A/ Unbalanced hydrogen bonding in the body of the water

B/ Balanced hydrogen bonding in the body of the water

C/ Balanced hydrogen bonding at the surface of the water

D/ Unbalanced hydrogen bonding at the surface of the water

23. Which one of the following pair of monosaccharides are trios’ sugar?

A/ Dihydroxyacetone and Ribulose                    B/ Glyceraldehyde and Ribulose

C/ Glyceraldehyde and Dihydroxyacetone          D/ Lactose and Maltose

24. Which one of the following dyes is used to stain chromosomes in dividing nuclei?  

A/ Haematoxylin            B/ Crystal violate         C/ Methylene blue           D/ Acetocarmine

25. Which of the following Ethiopian biologists is known internationally for developing a vaccine using genetic engineering to treat cattle disease rinderpest?  

A/ Dr Gebissa Ejeta                               B/ Professor Tilahun Yilma 

C/ Dr Melaku Worede                             D/ Professor Yalemtsehay Mekonnen

26. Which of the following Ethiopian biologists is known for their exemplary work in safeguarding the biodiversity and the traditional rights of farmers and communities to their genetic resources?

A/ Dr Tewolde Berhan Gebre Egziabher                       B/ Dr Zeresenay Alemseged

C/ Professor Ensermu Kelbessa                                     D/ Professor Sebsebe Demissew

27. Which one of the following types of cells is an unspecialized cell?

A/ Pancreas                  B/ Neuron              C/ Embryo              D/ Kidney

28. Which one of the following is incorrectly much in monomer-polymer pairing?

A/ Fatty acid – Lipid                                              B/ Amino acid – Protein

C/ Nucleotide - nucleic acids                                D/ Starch – Cellulose   

29. Which one of the following products is not produced as a result of microorganisms?

A/ Raw meat              B/ Alcohol              C/ Bread            D/ Vinegar

30. Which of the following is true about the cell structures and their respective functions?

A/ Cell wall: Support, protection                                B/ Chloroplasts: Site of cellular respiration

C/ Chromosomes: Cytoskeleton of the nucleus            D/ Lysosomes: Formation of ATP                

31. Which one of the following monosaccharides has a ketone functional group?

A/ Glyceraldehyde                B/ Galactose                 C/ Glucose                  D/ Fructose

32. From the macromolecule lipid found in the myelin sheath that surrounds nerve cells is:

A/ Triglyceride           B/ Sphingolipid             C/ Sterol            D/ Phospholipid

33. The bonds that link the monomers of carbohydrates and lipids respectively are:

A/ Covalent bond and Ether bond                  B/ Ester bond and Glycosidic bond 

C/ Ether and Covalent bond                            D/ Glycosidic bond and ester bond

34. Which one of the following is mismatched?

 A/ Glucose +Glucose = Maltose                  B/ Galactose +Fructose = Sucrose 

C/ Glucose + Fructose = Sucrose                   D/ Glucose +Galactose = Lactose 

35. The hydrophobic and hydrophilic region of the cell membrane contains respectively:

A/ Lipid and water                                                  B/ Hydrocarbon and Phosphate

C/ Phosphate and Hydrocarbon                              D/ Water and Lipid

36. When heated with Benedict’s solution, which carbohydrates does not show color change?

A/ Maltose            B/ Lactose               C/ Sucrose                D/ Reduced sugar

37. Which organelles contain their own genetic material and are important for endosymbiosis?

 A/ Vacuole and Lysosome                         B/ Chloroplast and Mitochondria

 C/ Nucleus and Cell membrane                 D/ Endoplasmic reticulum and Golgi body

38. From the following scientists, who first saw the living cells?

A/ Rudolf Virchow                             B/ Anton van Leeuwenhoek

C/ Robert Hooke                                 D/ Rene Dutrochet

39. The most common bacteria species used as vector to transfer target gene from one plant of another in order to change or modify the characteristics of plants

A/ Bacillus thurigiensis                            B/ Agrobacterium tumefaciens        

C/ Bacillus popilliae                                D/ Beauveria bassiana 

40. Instruction: Answer the question based on the following information about the solutions with different concentrations as Solution A = 0.9 M sucrose; Solution B = 0.2 M sucrose; Solution C = 0.5 M sucrose; If a potato tissue is immersed, solution C does NOT show any change in weight, which one could be the most probable reason?

A/ The solution is hypotonic to the potato cell

B/ The solution is isotonic to the potato cell

C/ The potato cell has more concentration of solute than the solution

D/ The solution is hypertonic to the potato cell

41. Which one of the following pair of organelles is not membrane-bound?

 A/ Golgi apparatus and Endoplasmic reticulum                    B/ Mitochondria and Chloroplast 

C/ Ribosome and Plasma membrane                                       D/ Nucleus and Lysosomes

42. Which one of the following is true about prokaryotic cells?

A/ Have cell wall made from murein                     B/ Have cell wall made from glycoprotein 

C/ Have linear DNA structure                                 D/ Has small ribosome with 80S

43. Which one of the following bioremediations methos is an example of in-situ bioremediation?

 A/ Biopilis                B/ Composting           C/ Biostimulation        D/ Treatment plant

44. Forensic science is important for solving crime and paternity cases by analyzing DNA, which type of gene is used for the amplification of the genetic material?

A/ Sense strand             B/ Non- sense strand            C/ Exon gene              D/ Coded gene

45. Which function of the microbes in bread making achieved mainly through the release of gas to produce porous and spongy products?

A/ Unconditioning                    B/ Conditioning         C/ Imparting       D/ Leavening

46. Which method used by biologists to separate cellular organelles for studying their properties?

A/ Dehydrogenation          B/ Polymerization         C/ Phosphorylation        D/ Cell fractionation

47. Over millions of years ancestral prokaryotic cells became more membranous and then their membrane around the cell became more and more folded. This would evolve into 

     A/ Golgi body         B/ Mitochondria        C/ Endoplasmic reticulum         D/Chloroplast

48. The genetic material (DNA) of bacteria cells is-------             

    A/ Linear & bound with histone                              B/ Linear & not bound with histone 

    C/ Circular & not bound with histone                      D/ Circular & bound with histone 

49. Various molecules in the membrane allow the cell to be recognized by hormones and the immune system (in animals) and growth regulator substances (in plants). These roles of the membrane are---

  A/ Cell signaling     B/ Boundary     C/ Cell communication      D/ Selective permeable

50. During the production of beer, wine and spirits the most common types of alcohol produced as a result of fermentation through microbial action is…

A/ Isopropanol alcohol          B/ Ethyl alcohol          C/ Amyl alcohol      D/ Isoamyl alcohol

51. Microorganisms are used for making various dairy products. From this milk product is the most common product produced by microbial action. Which pair of bacteria are commonly used for the production of yogurt?

A/ Streptococcus thermophilus and Lactobacillus bulgaricus  

B/ Streptococcus cremoris and Leuconostoc spp.  

C/ Lactobacillus plantarum and Lactobacillus bulgaricus

D/ Lactobacillus plantarum and Pediococcus cerevisiae

52. Which one of the following molecules could pass easily via the pore of the cell membrane?

    A/ Water soluble               B/ Non-polar                  C/ Charged                  D/ Hydrophilic

53. How much percent of the nucleus typically occupies the volume of a cell?

     A/ 50%                   B/ 40%                      C/ 20%                       D/10%

54. The functions of the rough endoplasmic reticulum and the Golgi body are related because:

A/ Proteins synthesized by the rough endoplasmic reticulum are modified by the Golgi body

B/ Proteins synthesized by the Golgi body are modified by the rough endoplasmic reticulum

C/ Lipids synthesized by the Golgi body are modified by the rough endoplasmic reticulum

D/ Lipids synthesized by the rough endoplasmic reticulum are modified by the Golgi body

55. Which one of the following microorganisms are most commonly used for the production of protein called Pruteen which is used as an animals feed?

A/ Fusarium venenatum                                  B/ Rhodopseudomonas capsulata

C/ Methylophilus methylotrophus                   D/ Saccharomyces cerevisiae

 

 

56. The mitochondrion, like the nucleus, has two or more membrane layers. How is the innermost of these layers different from that of the nucleus?

  A/ The two membranes are biochemically very different.

  B/ The inner mitochondrial membrane is highly folded.

  C/ The inner membrane of the mitochondrion is separated out into thylakoids.

  D/ The inner mitochondrial membrane is devoid of nearly all proteins

57. A cell has the following molecules and structures: enzymes, DNA, ribosomes, plasma membrane, endoplasmic reticulum, and mitochondria. It could be a cell from:

  A/ Plant or bacterium                                   C/ Animal, but not a plant

 B/ Plant, but not an animal                           D/ Plant or an animal

58. When biologists wish to study the internal ultrastructure of cells, they most likely would use

   A/ Light microscope                                            B/ Transmission electronic microscope                         

  C/ Scanning electron microscope                       D/ Compound microscope 

59. In the separation of homogenized cells using centrifugation, the primary factor that determines whether a specific cellular component ends up in the supernatant or the pellet is:

A/ Relative solubility of the component                   B/ Size and weight of the component

C/ Percentage of carbohydrates in the component    D/ Number of enzymes in the cell

60. The most common strategy that focus on the breading staple crop to have the high level of essential nutrients either through breading or genetic modification. 

A/ Biofortification                    B/ Food security            C/ Nutritional            D/ Food

61. Suppose Grade 9 students view the sample of the red blood cells under a light microscope. The magnification of the eyepiece lens is 10X and the object lens is 40X. One student takes an image of the blood cells from a light microscope and finds the size of the image is 20 mm. What is the actual size of the red blood cells in the sample?

A/ 0.05 mm              B/ 5 mm                C/ 0.02 mm                  D/ 2 mm

62. What is bioremediation?

A. The process in which a biological organism metabolizes a pollutant into a harmless compound.

B. The process of adding microorganisms to the environment.

C. The process of adding nutrients to the environment to neutralize pollutants.

D. The process of developing pollutants that can combat microorganisms.

63. The two domains to which prokaryotes are classified into are:

A. Bacteria and Protista                                  C. Bacteria and Archaea

B. Archaea and Eukarya                                 D. Eukarya and Monera

64. Which one of the following biological research center in Ethiopian widened doing research target on tuberculosis (TB), leishmaniasis, malaria, HIV/AIDS, and leprosy.

A/ Ethiopian Health and Nutrition Research institute          B/ Institute of Pathobiology

C/ Armauer Hansen Research institute                             D/ Institute of Agricultural Research

65. Which one of the following biological research center in Ethiopian practicing about conserving genes plants genes, plants, animals and micro-organisms which is very crucial for the management of the ecosystem.

A/ Institute of Biodiversity Conservation

B/ Institute of Agricultural Research

C/ Institute of Pathobiology

D/ Addis Ababa University Biology Department

66. Schistosomiasis or bilharziasis is a common parasitic disease. The spread of this disease is under controlled by the Ethiopia’s most renowned biologists Dr Aklilu Lemma using

A/ Koseret (Lippia abyssinica)                  B/ Enkoko (Embelia schimperi Vatke)

C/ Endod (Phytolacca dodecandra)           D/ Kosso (Hagenia abyssinica)

67. Dr Tewolde Berhan Gebre Egziabher is a biologist who researches into:

A/ HIV/AIDS                                    B/ Genetic engineering

C/ Human evolution                            D/ Environmental protection and diversity

68. Which one of the following is true about cell?

A/ There are organisms that are not composed of cell

B/ Some cells can be seen very easily with the naked eye without microscope

C/ All cells are invisible with our naked eye 

D/ All cells comes from preexisting cell by crystallization   

69. The biggest problem faced using light microscope is

A/ Low resolution but high magnification power

B/ High resolution but low magnification power

C/ Low resolution and low magnification power

D/ High resolution but high magnification power  

70. Which of the following is the main advantage of the electron microscope?

A/ It’s very expensive.

B/ Specimens are examined in a vacuum so must be dead.

C/ It needs a constant temperature and pressure.

D/ It gives a greatly increased magnification and resolution over the light microscope.

71. All living organisms are sensitive to changes in their surroundings. This property of living organisms is…

A/ Irritability           B/ Homeostasis            C/ Excretion        D/ Adaptation

72. What is the role of enzyme in bioremediation?

A. It can provide energy to the microorganisms involved in the process.

B. To transport the pollutant to designated treatment facility

C. To break down pollutants in small and less toxic molecules

D.  It serves as nutrient source for pollutant degrading microorganisms.

73. The cytoplasm is a liquid gel in which most of the chemical reactions needed for life take place and 70% of the component is

A/ Protein           B/ Water          C/ Salt              D/ Gases

74. The reliability of an experiment is increased by:

A/ Minimizing personal judgement                      B/ Working as quickly as possible

C/ Carrying out repeat experiment                       D/ Using the most appropriate apparatus

75. Which organelle has a three-dimensional system of tubules that spreads right through the cytoplasm and links the nucleus with the cell membrane?

A/ Mitochondria                B/ Endoplasmic reticulum         C/ Lysosome        D/ Cytoskeleton

76. ________ is a space in the cytoplasm filled with cell sap, a liquid containing sugars, mineral ions and other chemicals dissolved in water.

A/ Vacuole                B/ Ribosome               C/ Nucleolus         D/ Chloroplast 

77. Sea stars are broken apart by workers to save the clams they feed on, and then thrown back into the ocean. Often the numbers of sea stars are seen to double after this. This best explains

A. Regeneration            B. Fragmentation      C. Budding      D. parthenogenesis

 

78. What are the three phases of embryonic development in chronological order?

A. Blastula → Gastrula → Cleavage         B. Blastula → Cleavage → Gastrula

C. Cleavage → Gastrula → Blastula         D. Cleavage → Blastula → Gastrula

79. Genetically unique individuals are produced through ________.

A. Sexual reproduction               B. Parthenogenesis       C. Budding      D. Fragmentation

80. Why is sexual reproduction useful?

A. It completes in a very short period of time

B. It results in the rapid production of many offspring

C. It increases genetic diversity, allowing organisms to survive in an unpredictable Environment.

D. It needs less energy and leads to genetic variation in the offspring

81. External fertilization occurs in which type of environment?

A. Forest                     B. Aquatic                   C. Savanna                  D. Deep Soil

82. All living organisms are sensitive to changes in their surroundings. These characteristics of the living organism are referred to as?

A/ Movement             B/ Respiration             C/ Respond to stimulus        D/ Evolve over time

83. Which mating system involves a male mating with multiple females during a reproductive season?

A. Androgeny             B. Polygamy               C. Monogamy              D. Polygyny

84. Metamorphosis of insects is regulated through hormone

A. Pheromone                         B. Ecdysone                C. Thyroxine               D. Hypothalamus

85. ________ is a mission-oriented science that focus on the protection and restoration of biodiversity and the diversity of life on the earth. 

A/ Environmental Biology                   C/ Conservation Biology

B/ Developmental biology                   D/ Systematic Biology 

86. Which one of the following is not the importance of protecting natural resources?

A/ Protect wildlife and preserve it for future generations

B/ Improve water quality and air quality

C/ Preserve open and green spaces

D/ Increase exploitation of natural resources by humans

 87. ________ is a strategy of increasing the micronutrients level in food group through the uses of modern biotechnological techniques and traditional plant breading and agronomic practices.

A/ Biofortification              B/ Food security             C/ Food insecurity             D/ Nutrition

88. Based on FDA, The condition when the person become “too short” due to malnutrition is-----

A/ Good nutrition       B/ Over nutrition         C/ Stunting             D/ Wasting  

89. Which one of the following is not the major objectives to be the organisms are being genetically modified?

A/ Increase yield and to obtain novel products  

B/ Enhance of contamination from industrial, and municipal wastes.

C/ Diagnosis and treatment of diseases 

D/ Production for pharmaceuticals and gene therapy

90. Which one is not the reason for microorganisms are a preferable sources of single cell protein?

A/ Have short life cycle

B/ Produce better protein in terms of quality and the quantity 

C/ Not affected by fluctuation of weather condition

D/ Can be grow in cheap media in small amount

91.  In developed country, the single cell protein used as an animal feed called Pruteen is produced from-----? 

A/ Methylophilus methylotrophus                   C/ Spirulina

B/ Fusarium venenatum                                   D/ Chlorella

92. Yogurt is one of the common fermented food product produced as a result of fermentation carried out by a starter culture-----------?

A/ Streptococcus pneumonia and Lactobacillus acidophilus

B/Streptococcus thermophilus and Lactobacillus bulgaricus

C/ Streptococcus cremoris and Leuconostoc sp.

D/ Lactobacillus plantarum and Pediococcus cerevisiae

93. Which one of the following is the product of homo-lactic acid fermentation?

A/ Glucose      B/Ethanol        C/Lactic acid            D/ Carbon dioxide 

94. In alcoholic production cereal grains are malted. So, malt is----?

A/ Partially germinated barely           C/ Crushed cereals

B/ Liquid extract                                D/ Fermented grains

95.  The alcoholic content of various alcoholic beverage is enhanced by the process of-----?

A/ Microbial action              B/ Fermentation            C/ Distillation        D/ Increase substrate

96. Which one of the following is true about lager beer?

A/ Produced by top fermentation              C/ The starter culture is Saccharomyces cerevisiae

B/ Fermented by low temperature               D/ The alcohol content is reaches up to 10%

97. In bakery production, the process of releasing of gas (CO2) to produce porous and spongy product via microbial action is------?

A/ Leavening          B/ Imparting             C/ Baking           D/ Conditioning

98. Animals can be genetically modified (transgenic animals) for the purpose of all except?

A/ Enhancing reproductive performance           C/ Increasing growth rate

B/ Improving carcass composition                     D/ Increasing disease susceptibility

99. _____ is method of transferring a fragments of tissue from an animal or plant to an artificial environment for production of disease-free and high quality products.

A/ Transplantation             B/ Tissue culture       C/ Mixed culture           D/Pure culture

100. One of the major application of biology in human health and wellbeing’s are the production of antibiotics. This products are produced due to……?

A/ Cooperation for limited nutrient                 C/ Antagonism for inadequate of food resources

D/ Synergism for the presence of space           D/ The occurrence of oxygen abundance

101. In the lysogenic cycle of virus reproduction:

A/ The viral DNA becomes incorporated into the host cell’s DNA

B/ Causing the cell to lysis and move the viruses out of the host

C/ Eventually the viral DNA becomes activated and causes the release of viral proteins

D/ Leads the rupture of the host cell

102. Bacterial populations can develop a resistance to antibiotics. Which factors listed below do not contribute to emerging antibiotic resistance?

A/ Random mutation in the bacterial population

B/ The repeated use of the same antibiotic on a bacterial population

C/ Careful selection of the most effective antibiotic to use on a bacterial population

D/ People not completing a course of antibiotics so that some bacteria survive

103. The reason that Gram negative bacteria are more toxic than Gram negative bacteria are due to the fact that Gram negative bacteria contain?

A/ Lipoteichoic acid               B/ Lipoprotein         C/ Lipopolysaccharide       D/ O-Antigen

104. On the evolutionary lines, which one of the following microorganisms do not have distinctive domain and kingdom?

A/ Viruses                  B/ Fungi                  C/ Helminthes              D/ Protozoa

105. Which one of the following external structure of bacteria used for prevent the bacteria from dehydration?

A/ Pilli                   B/ Fimbriae             C/ Glycocalyx                    D/ Inclusion body  

106. In a bacteria shape arrangement when many cocci are joined together and form clustered structure called------

A/ Streptobacilli               B/ Streptococcus          C/ Staphylococcus           D/ Sarcina

107. The rigid layer of the bacteria cell, which is composed of N-acetylglucosamine and N-acetylmuramic acid linked by peptide bridge is---------

A/ Peptidoglycan                 B/ Chitin                  C/ Polypeptide               D/ Cellulose

108. Based on nutritional requirements, nearly all pathogenic microorganisms are grouped under—

A/ Chemolithoheterotroph                                 B/ Photolithoautotroph

C/ Chemoorganoheterotroph                             D/ Chemolithoautotroph 

109. When the archaea bacteria that can survive and live in very saline environments (like the Dead Sea) is. 

A/ Hyperthermophile            B/ Cryophile        C/ Psychrophile               D/ Halophile 

110. Archaea have become the source of enzymes harnessed as the basis for the amplification of the DNA in a technique called Polymerase chain reaction (PCR). This enzyme is an essential part of the development of molecular biology which obtained from

A/ Staphylococcus aurous      B/ Methanogen     C/ Thermus aquaticus    D/ Toxoplasma gondii 

111. In a viral replication cycle the assembly of newly synthesized viral components into complete viruses in-----.

A/ Biosynthesis stage         B/ Release stage       C/ Adsorption stage      D/ Maturation stage

112. Which one of the following statements is odd about the idea of Koch's postulates?

A/ The suspected microorganisms must be cultivated in a pure culture in vivo.

B/ Disease-causing organisms must always be present in diseased animals but not in healthy animals

C/ The isolated organism must cause the same disease when inoculated into a healthy animal

D/ The same microorganism must be reisolated from the newly infected animals and cultured again in the laboratory

113. Which one of the following is not pure culture techniques?

A/ Pour plate method              

B/ Streak plate method                 

C/ Spread plate method           

D/ Serial dilution

114. Which one of the following methods of controlling the growth of microorganisms by boiling and rapidly colling the food without affecting the nutritive value of the food?

A/ Tyndallization             B/ Sterilization             C/ Pasteurization       D/ Aseptic technique

115. A type of dormant cell that is metabolically inactive, cannot grow and develop, and is extraordinarily resistant to heat, ultraviolet light, and toxic chemicals is------- 

A/ Plasmid                 B/ Spore                 C/ Endospore               D/ Vegetative

116. Which one is true about protoplast and spheroplast?

A/ Protoplast is unbounded by any membrane

B/ Spheroplast has an inner membrane and an outer membrane

C/ Spheroplast are bounded by a single membrane

D/ Protoplast has an inner membrane and an outer membrane

117. Which one of the following is unique about the characteristics of archaea?

A/ Have cell membrane with unbranched hydrocarbon chains

B/ Have cell wall composed of pseudopeptidoglycan

C/ Many are found in harsh environments

D/ The initial amino acid in their polypeptide chain is methionine

118. The most common types of fungi called yeast that used for the production of fermented food products is grouped under…

A/ Basidomycota          B/ Zygomycota          C/ Chytridomycota             D/ Ascomycota

119. The unprotected dust-like spore produced by some fungi by the process of sporulation is…

A/ Conidiophore             B/ Arthrospore             C/ Sporangiospore            D/ Blastospore

120. In sexual reproduction of fungi, opposite mating types come together and fuse. So, the fusion cells represent---  

A/ Fruiting body           B/ Heterokaryon              C/ Basidiocarp                  D/ Basidiomycete

121. One of the harmful aspect of fungi is producing toxin that cause diseases for animal and human. Which one of the following toxins is produced by mushroom? 

A/ Ergot              B/ Aflatoxin               C/ Mycetism                   D/ Sclerotium

122. Which one of the following dermatophytosis most commonly affects hairless skin of human?

A/ Tinea capitis                 B/ Tinea corporis              C/ Tinea pedis             D/ Tinea unguium

123. Which one of the following structures is found only in enveloped viruses?

A/ Genetic material              B/ Capsid              C/ Matrix protein           D/ Spike

124. Which one of the following is the harmful aspect of fungi?

 A/ Studying cancer and aging within a simple cell

B/ Deterioration of fabrics, leather, electrical insulation and other manufactured goods

C/ Production of citric, oxalic, gluconic and itaconic acid

D/ Manufacture of immunosuppressive drug called cyclosporin

125. Superficial mycoses are fungal infections of the outermost areas of the human body. Which one of the following common genera of fungi cause this infection?

A/ Microsporum         B/ Aspergillus             C/ Mycotoxicoses           D/ Candidiasis

126. Which one of the following common protozoa is correctly matched with their class?

A/ Euglena ------------ Rhizopodea               B/ Trypanosoma------ Mastigophora

C/ Paramecium-------Mastigophora              D/ Giardia----------- Ciliata

127. In sexual reproduction of protozoa, genetic variation results from a sexual process in which two individuals exchange haploid micronuclei but do not reproduce. This type of genetic variation comes as result of ----  

A/ Meiosis        B/ Mitosis           C/ Micronuclear fusion             D/ Conjugation 

128. Food is digested in the vacuole of protozoa after the food enters the cell. When the process is completed the waste products are excreted using a process called------

A/ Exocytosis                 B/ Vesicle             C/ Ingestion                     D/ Egestion                    

 129. Which one of the following common structures is found in almost all viruses? 

A/ DNA              B/ RNA                  C/ Capsid               D/ Tail fiber 

130. Which one of the following methods is not used for studying viruses?

A/ Compound microscope      B/ Electron microscope        C/ Centrifugation      D/ Filtration

131. All of the following statement is true about DNA and RNA viruses except?

A/ Both DNA and RNA are found in a single virus as a genetic material 

B/ RNA mutation rate is higher than the DNA mutation rate

C/ DNA viruses are mostly double-stranded while RNA viruses are single-stranded

D/ The disease caused by RNA viruses are more toxic than DNA viruses

132. Which of the following is collective name for all bacteria with spherical shape?

A/ Bacilli          B/ Cocci           C/ Streptococci             D/ spirochetes

133. Which one of the following is the correct increasing order of thus most common element found in the living system in percentages?

A/ Nitrogen, Oxygen, Phosphorus, Hydrogen, Carbon

B/ Phosphorus, Nitrogen, Carbon, Oxygen, Hydrogen

C/ Oxygen, Hydrogen, Phosphorus, Nitrogen, and Carbon

D/ Nitrogen, Phosphorus, Oxygen, Carbon, Hydrogen

134. Which one of the following is the correct path of breathing?

A/ Bronchus ------Bronchiole --- Trachea ------ Alveoli

B/ Trachea------Bronchus------Bronchiole---Alveoli

C/ Trachea ------Bronchiole------Bronchus ---Alveoli

D/ Bronchiole ------Bronchus --------- Trachea ------ Alveoli

135. Which one of the following conditions will occur during the inhaling of oxygen into the lung?

A/ Chest cavity become increase

B/ Diaphragm muscles relax and becomes dome-shaped

C/ Diaphragm muscles contract and becomes dome-shaped

D/ Chest cavity become increase

136. Which one of the following structures of our respiratory system also called “voice box”.

A/ Pharynx      B/ Epiglottis    C/ Larynx       D/ Gullet

 

137. The only viruses that affect bacteria is-------------?

A/ Corona virus         B/ Herpes simplex virus         C/ Phage virus          D/ Zika virus

138. The correct path of human digestive system is

A/ Mouth--stomach---- gullet— small intestine---large intestine--- rectum--- anus

B/ A/ Mouth--- gullet—stomach---- small intestine---large intestine--- rectum--- anus

C/ Mouth—stomach--- gullet ---large intestine---- small intestine --- rectum--- anus

D/ Mouth--- gullet—stomach ---large intestine---- small intestine --- rectum--- anus 

139. Chemical digestion of carbohydrates begins in the

A/ Esophagus           B/ Stomach         C/ Duodenum        D/ Oral cavity

140. Which one of the following wastewater treatments is an example of biological treatment?

A/ Primary wastewater treatment             B/ Secondary wastewater treatment

C/ Tertiary wastewater treatment            D/ Quaternary wastewater treatment

141. ------is the amount of dissolved oxygen needed by aerobic biological organisms to break down organic material present in a given wastewater sample at certain temperature over a specific time period.

A/ BOD             B/ COD           C/ Treatment plant           D/ Flocs

142. Which one of the following alcoholic beverages has high alcohol content?

A/ Tella         B/ Wine           C/ Beer            D/Spirits

143---- is a process that relies on microorganisms and plants and/or their derivatives to degrade or alter environmental contaminants as these organisms carry out their normal life functions.

A/ Bioremediation             B/ Bio fabrication          C/ Bio stimulation     D/ Bioaugmentation

144. Any antimicrobial agents that apply on living tissue/body surface is

A/ Sterilization           B/ Disinfectant          C/ Antiseptic       D/ Decontamination

145. -------- is the minimum amount of time in which all microbes in a liquid culture are killed at a given temperature.

A/ Thermal Death Point                                 B/ Thermal Death Time

C/ Low-temperature pasteurization              D/ High-temperature pasteurization

146. --- is the sterilization of inoculating loop or wire, tip of forceps, etc., using Bunsen burner till it become red hot.

A/ Incineration   B/ Flaming            C/ Hot air oven           D/Moist heat sterilization

147. Which one of the following methods is carried out over a period of 3 days and requires a chamber to hold the materials and are exposed to free-flowing steam at 100°C for 20 minutes, for each of the three consecutive days.

A/ Sterilization              B/ Pasteurization         C/ Incineration          D/ Tyndallization

148. Which one of the following bacterial characterization methods is morphological test?

A/ Catalase test         B/ PCR test          C/ Shape test           D/ Fermentation test

149. ------- is a method of producing identical copies of genes, cells, or organisms.

A/ Cloning                                                  B/ Genetic engineering

C/ Recombinant DNA technology             D/ Breeding

150. -----is the use infectious agents such as bacteria, viruses, and fungi with the intent to kill or incapacitate humans, animals or plants as an act of war.

A/ Chemical attack          B/ Criminal          C/ Biological weapons       D/ Pesticides

151. When many spherical shapes of bacteria are arranged in straight light is forms

A/Spirochetes         B/ Coccobacilli       C/ Streptococcus         D/ Staphylococcus

152. During staining, Gram negative bacteria retain the color of

A/ Crystal violate        B/ Safranine         C/ Iodine             D/ Malachite green

153. Bacteria can reproduce asexually by

A/ Binary fusion        B/ Fragmentation         C/ Binary fission         D/ Parthenogenesis

154. The horizontal gene transfer occur in the bacteria by uptake foreign DNA from the external environment is---

A/ Transformation       B/ Conjugation       C/ Transduction          D/ Transcongugation

155. The type of archaebacteria that used in the production of the major biogas component is

A/ Extreme thermophile      B/ Hyperthermophile     C/ Halophile       D/ Methanogen

156. Mycorrhizal fungi are used in agricultural for increasing soil fertility by live with the symbiosis with plant root. This fungus is grouped under

A/ Basidomycota        B/ Chytridomycota        C/ Zygomycota     D/ Glomeromycota

157.  Which one of the following statements is incorrect about microorganisms?

A/ Are too small but can be seen clearly by the unaided eyes

B/ Are too small and cannot be seen without magnification

C/ Microorganisms can be both prokaryotic and eukaryotic 

D/ Majority of them are harmful and few of them are useful

158. Which one of the following is not the function of protein?

A/ Speed up a chemical reaction                     B/ Control of metabolism                                       

C/ Act as a coenzyme                                       D/ As a structural component

159. Which one of the following statements is true about the chemical reaction catalyzed by an enzyme?

 A/ Enzyme is consumed in the reaction                           B/ Rate of reactant formation increases

 C/ Increases the collusion of reactant molecules               D/ The energy barrier increases

160. Which one of the following is incorrect about protein structures?

A/ Each protein has a unique primary structure and so has a unique configuration 

 B/ The primary structure determines the type and position of amino acid 

 C/ The secondary structure determines the tertiary structure and shape of the protein molecule

D/ The secondary structure determines the formation of ionic and hydrogen bonds

161. The enzyme that catalyzes the addition /removal of atoms to/from a double bond is?  

A. Lyases                B. Hydrolases            C. Ligases              D. Transferases

162. The end product of the pathway blocks the functioning of the whole enzyme by binding to the allosteric site of the first enzyme. This mechanism of enzyme regulation is:

   A. Covalent modification                         B. Allosteric regulation 

   C. Allosteric activation                            D. Zymogen activation

163. Which one of the following is true about the induced fit model of enzymes?

    A. During binding the substrate shows conformational changes to accommodate the enzyme

    B. Naturally, the substrate exactly fits with the active site

    C. By nature the active site of the enzyme is flexible 

     D. The substrate and active site are always complimentary      

164. Enzymes are classified based on the type of reactions that they catalyze. So how many classes of enzymes are there in the Enzyme Commission (EC) classification? 

A/ 5                B/ 8                   C/ 4               D/ 6

165. Which one of the following enzymatic factors could increase the activity of the enzyme steadily or linearly?

A/ Temperature      B/ Substrate concentration            C/ pH           D/ Enzyme concentration

166. Zinc, an essential trace element for most organisms, is present in the active site of the enzyme carboxypeptidase. The zinc most likely functions as a(n)

     A/ Competitive inhibitor of the enzyme      B/ Noncompetitive inhibitor of the enzyme

    C/ Allosteric activator of the enzyme          D/ Cofactor necessary for enzyme activity

167. Which of the following statements regarding enzymes is true?

      A/ Enzymes decrease the free energy change of a reaction

      B/ Enzymes increase the rate of a reaction

      C/ Enzymes change the direction of chemical reactions

      D/ Enzymes are permanently altered by the reactions they catalyze

168. Ms. Alemitu has a baby named Kumbi. She believes one of two men can be the father of Kumbi. A paternity test was done and the results were as shown in the figure below. Which man is the father of her baby?

     A/ Dad 1             B/ Dad 2               C/ None of them          D/ Both could be father

169. Sucrose is a disaccharide, composed of the monosaccharide’s glucose and fructose. The hydrolysis of sucrose by the enzyme sucrase results in: -

A/ Production of water from the sugar as bonds are broken between the glucose monomers

B/ Utilization of water as a covalent bond is formed between glucose and fructose to form sucrase

C/ Breaking the bond between glucose and fructose and forming new bonds from the atoms of water

D/ The release of water from sucrose as the bond between glucose and fructose is broken.

170. How does a non-competitive inhibitor decrease the rate of an enzyme reaction?

A/ By acting as a coenzyme for the reaction               

B/ By changing the structure of the enzyme

C/ By changing the free energy change of the reaction

D/ By binding at the active site of the enzyme             

171. In the nitrogen cycle, the plants do not use inert nitrogen gases directly from the atmosphere rather they absorb nitrate, so, which one of the following bacterial species is used for converting nitrite into nitrate?

A/Nitrosomonas               B/Nitrobacter                   C/ Azotobacter                 D/ Rhizobium

172. Which one of the following statements is odd about irreversible inhibitions?

A/ Cannot be removed by simple dialysis or super-filtration

B/ The inhibitors are covalently bound to the essential groups of enzymes

C/ Pesticide and heavy metal poisoning are bound to target molecule reversible

D/ Cause a partial loss or complete loss of the enzymatic activity    

173. In the lysogenic cycle of virus reproduction:

A/ The viral DNA becomes incorporated into the host cell’s DNA

B/ Causing the cell to lysis and move the viruses out of the host

C/ Eventually the viral DNA becomes activated and causes the release of viral proteins

D/ Leads the rupture of the host cell

174. Bacterial populations can develop a resistance to antibiotics. Which factors listed below do not contribute to emerging antibiotic resistance?

A/ Random mutation in the bacterial population

B/ The repeated use of the same antibiotic on a bacterial population

C/ Careful selection of the most effective antibiotic to use on a bacterial population

D/ People not completing a course of antibiotics so that some bacteria survive

175. One of the uses of microorganisms in genetic engineering is producing transgenic plants, which method is used for inserting the target gene to make transgenic plants specifically in maize, tobacco, carrots, soybean, and apple?

A/ Plasmid                    B/ Agrobacterium tumefaciens             C/ GMO               D/ Gene gun

176. In Gram staining techniques, if the unknown bacteria retain the color of the primary dye after staining is more probably it will be

A/ Gram Variable            B/ Gram-negative          C/ Gram intermediate       D/ Gram-positive         

177. In the systematic naming of the enzyme, assume an enzyme is designed as E.C.2.5.8.1. Which one of the following correctly describes this enzyme?

A/ 5- shows to which main class the enzyme belongs

B/ 8- gives a sub-subclass to which the enzyme belongs

C/ 1- a reference of Enzyme Commission

D/ 2- indicates a sub-class in which the enzyme belongs  

178. Which one of the following statements best explains the idea of the key-and-lock model of enzyme action?

A/ The model explains the formation of an intermediate state 

B/ Shape of the substrate is complementary to the allosteric site of the enzyme

C/ Attached the substrate to an enzyme produces a shape change

D/ Attached substrate the enzyme complex enters into a transition state  

179. Which one of the following is true about apoenzyme?

A/ Cofactors that give an enzyme its catalytic activity

B/ Inorganic molecules that bind loosely with the cofactor

C/ Protein that combine with cofactors to form active enzyme 

D/ Organic molecule and many are derived from vitamin

180. How does competitive inhibitor affect enzymes catalytic reactions?

A/ Binding to the allosteric site of enzyme                        B/ Denature the active site of enzyme

C/ Producing conformational change to the enzyme          D/ Blocking the active site of enzyme                

181. On the evolutionary lines, which one of the following microorganisms do not have distinctive domain and kingdom?

A/ Viruses                  B/ Fungi                  C/ Helminthes              D/ Protozoa

 182. Which one of the following statements is correct about bacteria?

A/ Are unicellular and eukaryotic cell without nucleus

B/ Are ubiquitous

C/ All bacteria have cell wall made up of peptidoglycan

D/ Few photosynthetic bacteria have true chlorophyll

 

183. Which one of the following external structure of bacteria used for prevent the bacteria from dehydration?

A/ Pilli                   B/ Fimbriae             C/ Glycocalyx                    D/ Inclusion body  

184. The rigid layer of the bacteria cell, which is composed of N-acetylglucosamine and N-acetylmuramic acid linked by peptide bridge is---------

A/ Murine (peptidoglycan)            B/ Chitin                  C/ Polypeptide               D/ Cellulose

185. Based on nutritional requirements, nearly all pathogenic microorganisms are grouped under—

A/ Chemolithoheterotroph                                 B/ Photolithoautotroph

C/ Chemoorganoheterotroph                             D/ Chemolithoautotroph 

186. Which one of the following bacterial diseases is not transmitted by sexual intercourse?

A/ Gonorrhea               B/ Neisseria              C/ Syphilis                 D/ Leprosy

187. When the archaea bacteria that can survive and live in very saline environments (like the Dead Sea) is. 

A/ Hyperthermophile            B/ Cryophile        C/ Psychrophile               D/ Halophile 

188. Which one of the following is unique about the characteristics of archaea?

A/ Have cell membrane with unbranched hydrocarbon chains

B/ Have cell wall composed of pseudopeptidoglycan

C/ Many are found in harsh environments

D/ The initial amino acid in their polypeptide chain is methionine

189. The most common type of fungi called yeast that is used for the production of fermented food products is grouped under…

A/ Basidomycota          B/ Zygomycota          C/ Chytridomycota             D/ Ascomycota

190. The unprotected dust-like spore produced by some fungi by the process of sporulation is…

A/ Conidiophore             B/ Arthrospore             C/ Sporangiospore            D/ Blastospore

191. One of the harmful aspects of fungi is producing toxins that cause diseases for animals and humans. Which one of the following toxins is produced by mushrooms? 

A/ Ergot              B/ Aflatoxin               C/ Mycetism                   D/ Sclerotium

192. Which dermatophytosis is most commonly affects the hairless skin of humans?

A/ Tinea capitis                 B/ Tinea corporis              C/ Tinea pedis             D/ Tinea unguium

193. All of the following statements are true about the microorganism protozoa except?

A/ Unicellular organisms without a cell wall

B/ Can be free-living or parasitic

C/ Some protozoa can synthesize their food by photosynthesis

D/ Most of them are anaerobic

194. Which one of the following types of protozoa reproduction is from schizogony and common in Plasmodium that causes malaria disease?

A/ Binary fission                B/ Budding              C/ Multiple fission         D/ Transverse fission

195. The disease African sleeping sickness is transmitted by a vector Tsetse fly (Glossina) and shows symptoms like fever, and joint pains followed by neurological disorder) are caused by

A/ Plasmodium falciform                               B/ Leishmania donovani

C/ Toxoplasma gondii                                     D/ Trypanosoma brucei

196. Which one of the following statements is odd about viruses?

A/ Can be grown on cell-free media                        B/ Some viruses contain enzymes

C/ Can be seen with an electron microscope           D/ Are unaffected by antibiotics

197. The outer membrane portion of the cell envelope of certain gram-negative bacteria, which is a toxin to humans when solubilized is

A/ Endospore                  B/ Endotoxin               C/ Exospore                D/ Exotoxin

198. Both protoplasts and spheroplasts are altered forms of plant, bacterial, or fungal cells from which the cell wall has been partially or completely removed. Which one is true about protoplast and spheroplast?

A/ Protoplast is unbounded by any membrane

B/ Spheroplast has an inner membrane and an outer membrane

C/ Spheroplast are bounded by a single membrane

D/ Protoplast has an inner membrane and an outer membrane

1999. Mr. X’s white blood cells, intestine, and skin are highly affected as a result the skin ulcer, mucocutaneous, complications, and visceral, diseases (hepatosplenomegaly). Based on these symptoms he is sure that the disease is transmitted by the vector Sand fly (Phlemotomus). So, what is the name of this disease?

A/ Toxoplasmosis                                           B/ Leishmaniasis (kalaazar)

C/ African sleeping sickness                           D/ Trichomoniasis

200. A doctor takes a certain patient's stool sample. After examination, the clinical features of the disease look like tissue destruction, intestinal cramps, ulceration, and blood. Based on these features the doctor identified the disease as caused by protozoa and concluded that this disease is transmitted by ingestion of fecally contaminated food or water, a disease associated with poverty, homosexual men, and migrant workers. So, the causative agent of this disease is-----------

A/ Trypanosoma brucei                               B/ Giardia lamblia

C/ Entamoeba histolytica                             D/ Trichomonas vaginalis

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